Fig. 2
From: Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry
a T4, ФX174, and lambda virions are partitioned into droplets with TaqMan primers and probe specific for T4. After thermal cycling, the T4 sample has TaqMan signal while the ФX174 and lambda negative controls have no signal, demonstrating that digital droplet PCR specifically detects target viruses. b The fraction of TaqMan positive droplets in digital PCR for T4 is closely related to the input T4 concentration, showing that digital droplet PCR quantitatively measures viral concentration. Error bars indicate the standard deviation for triplicate measurements