Fig. 1
From: Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry
PCR-Activated Virus Sorting (PAVS) workflow. Virus suspensions are encapsulated with PCR reagent and probe in double emulsion droplets, then thermal cycled and stained with SYBR Green. FACS recovers fluorescent droplets containing the viral species of interest, generating an enriched sample that is ready for downstream processing