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Table 1 Primer sequences used for flavivirus, yellow fever, West Nile, dengue consensus and sero type specific RT-PCR reactions

From: Detection of dengue virus serotypes 1, 2 and 3 in selected regions of Kenya: 2011–2014

Primer Sequence Base pair size of amplified product Reference
FU1
CFD3
5′- TAC AAC ATG ATG GGA AAG AGA GAG AA-3′
5′- GTG TCC CAG CCG GCG GTG TCA TCA GC-3′
260 Flavivirus [46]
KP7
KP81
5′-GCA GAG TGA TCG ACA GCC G-3′
5′-CCA CCA GAC CAT TCG GCA TG-3′
258 West Nile [47]
YF7 R
CAGF
5′- AAT GCT CCC TTT CCC AAA TA- 3′
5′- CGA GTT GCT AGG CAA TAA ACA CAT TTG GA-3
670 Yellow Fever [48]
D1
D2
5′-TCAATATGCTGAAACGCGCGAGAAACCG-3′
5′-TTGCACCAACAGTCAATGTCTTCAGGTTC-3′
511 Dengue consensus [49]
TS1 5′-CGTCTCAGTGATCCGGGGG-3′ 482 (Dl and TS1) DENV1 [49]
TS2 5′-CGCCACAAGGGCCATGAACAG-3′ 119 (Dl and TS2) DENV2 [49]
TS3 5′-TAACATCATCATGAGACAGAGC-3′ 290 (Dl and TS3) DENV3 [49]
TS4 5′-CTCTGTTGTCTTAAACAAGAGA-3′ 392 (Dl and TS4) DENV4 [49]