Fig. 3

CHIKV release from 293T cells. 293T cells were exposed to either CHIKV T-SBY or V-181 for 2 h at a MOI of 10. Unbound virus was removed and washed extensively. Supernatants were collected for determination of viral loads (a) and viral RNA (b) at early (0, 2, 4, 6, and 12 h) and late (1, 2, 3, 4, 7, 10 d) infection using standard plaque assay and real time PCR as described in Materials and Methods. Results are expressed as viral titer (PFU/ml) (a) and viral RNA copies/ml (b). The bars represent the mean values and the error bars represent the SEM values from three independent experiments. Asterisks (*) indicate p < 0.05