Fig. 2

Multiple sequence alignment of CHIKV E1 envelope glycoprotein (E1). The 1,317 base pair CHIKV E1 envelope glycoprotein gene from the isolated CHIKV was amplified by PCR. Comparison of the E1 nucleotide sequence using NCBI BLASTX revealed a close match with the E1 protein in the CHIK/SBY8/10 isolate from Indonesia (GenBank accession number AB678677; T-SBY). Amino acid sequences of the E1 from T-SBY were aligned with E1 from the 181/clone 25 vaccine strain (GenBank accession number L37661; V-181) and its parental strain AF15561 (GenBank accession number EF452493) [27] using ClastalW2 software [24]. Asterisks (*) indicate completely identical amino acids. An alteration of amino acid residues is shaded in grey. Single (.) and double dot (:) represent semi-conservative and conservative amino acid substitution