Fig. 3

The mutated phenotype of UL7-MU is complemented by expression of the UL7 gene in cells. a The lysates of Vero cells transfected with the pcDNA3-UL7 plasmid were separated on a 12 % polyacrylamide gel, transferred to a PVDF membrane, and immunoblotted with UL7 antibody. The position of the UL7 protein is indicated. b Morphological features of plaques formed by the complemented UL7-MU and WT viral strains. Cells were infected at 24 h post-transfection with UL7-MU or WT virus at an MOI of 1. The supernatants and cells of the complemented virus were harvested after CPE was observed, followed by plaque assays. Vero cells were infected with MU-complemented P1, WT-control P1, MU-complemented P2, WT-control P2 or HSV-1 WT at an MOI of 0.00001. The cells were fixed and stained 3 d p.i. c Dynamic profiles of the MU-complemented P1 and MU-complemented P2 viral strains at 37 °C. Vero cells were infected with the MU-complemented P1 (filled circles), MU-complemented P2 (filled triangles) or HSV-1 WT (open boxes) at an MOI of 1. The supernatants and cells were harvested at the indicated time points, and the lysates were titered on Vero cells