Fig. 2

Expression of p53 and its downstream proteins in cells latently infected with HIV-1. a The expression levels of p53 and phosphorylated p53 at Ser 15 (p-p53) in latently infected and uninfected cells were measured in the absence (upper panel) or presence of 200 μM of 5-FU for 12 h (lower panel). b The levels of expression of p53 and its downstream proteins were measured in latently infected ACH2 and NCHA2 upon treatment of 200 μM of 5-FU for 24 h. c Western blotting was used to analyze the p53 and p-p53 levels in J1.1 cells electroporated with p53 expression plasmid upon 5-FU treatment (upper panel). Apoptosis of the cells was measured as Fig. 1a. Total apoptotic cells are shown graphically with statistical analysis. The data are shown as mean ± SD (n = 3). *p <0.05, **p <0.01 compared with cells untreated with the drug