Fig. 1

5-FU treatment-induced apoptosis of cells latently infected with HIV-1. a The cells were treated with 5-FU at the indicated concentration for 24 h. After treatment, the cells were measured using flow cytometry. The number of apoptotic cells is shown as a percentage in each plot (upper panel). Total apoptotic cells are shown graphically with statistical analysis. The data are shown as mean ± SD (n = 3). **p <0.01 compared with uninfected A3.01 cells (lower panel). b The cells were treated with 400 μM of 5-FU for 48 h, and then lysed with RIPA buffer. Western blotting was used to analyze the protein levels from the cell lysates using antibodies against caspase 3, cleaved caspase 3, PARP and β-actin as a loading control