Table 1 nested primers used to detect the positive sample of HPV-ZJ01 and amplify the gaps between contigs
Primers’ name | Sequence (5′to 3′) | Primers′ name | Sequence (5′to 3′) |
|---|---|---|---|
Detection FO | AAATGAAACGCGGCAAGATCC | Primer4 FI | TGGCAAAAAGTGCAAGGAACTG |
Detection RO | TCGGTTGCTCTTGGACTACG | Primer4 RI | AAGGCATACAGCATTCTACTCC |
Detection FI | CGCGGCAAGATCCTGCAAATCA | Primer5 FO | AAGGGGACACATGGGCAGAT |
Detection RI | GCTGTGTGCCTTCGTACCGGG | Primer5 RO | GCGGGAAACTGAGCCTAAGAA |
Primer1 FO | GCTTGATGACTTTTGTTCTCACT | Primer5 FI | AGGTACTGGTAGGGGGTCTG |
Primer1 RO | TAAGTGGACCTCCCATGCTG | Primer5 RI | CTTGGACAGGCGTGCTAGAT |
Primer1 FI | AGGATCTTGCCGCGTTTCATT | Primer6 FO | TCTTAGGCTCAGTTTCCCGC |
Primer1 RI | TAAGTGGACCTCCCATGCTG | Primer6 RO | AGTCTGTGGGCTTAATAGGCG |
Primer2 FO | GTCAGCATGGGAGGTCCACT | Primer6 FI | CCGCCTTTGAACCAGATGTC |
Primer2 RO | TGCTGCATTAGTATCAGTTTCTGC | Primer6 RI | CCAACCTGCAAGCCACTTCT |
Primer2 FI | ATGAGAATGTGGATGCTGAGGA | Primer7 FO | TCTGCAATGACCACCAGAAGT |
Primer2 RI | AGCCCACTGTATCATTCGAGAC | Primer7 RO | AGAGAACCACTAGGTGAAGCAA |
Primer3 FO | GTGACAGATGTAAAACCGAAGG | Primer7 FI | GGCCTGTCCATTTTTATCAGGA |
Primer3 RO | TGCCCATTTGAATAGCTTGCTT | Primer7 RI | CACTGGAAGGTGTTGAATTTCC |
Primer3 FI | GTGACAGATGTAAAACCGAAGG | Primer8 FO | GAGAGCTCAAGGACCAAACAA |
Primer3 RI | AGCTTGCTTAGCATTATTTTCAGAT | Primer8 RO | AAGGATGTCCAGATGTGACTG |
Primer4 FO | GCCTTGCCTTATCCTTGTTGG | Primer8 FI | GAGAGCTCAAGGACCAAACAA |
Prime4 RO | AGCACGTTTATTTCTACCAGCTC | Primer8 RI | TAACAAGCACCAAACAACTCCC |