Fig. 3
BTas transactivation of LTR and IP. a HEK293T cells (2.5 × 105) were transfected with 20 ng pGL3basic, BFV-3'LTR-B-Luc, pBFV-3'LTR-Y-Luc, pBFV-5'LTR-B-Luc or pBFV-5'LTR-Y-Luc together with 100 ng pCDNA3.1+, BTas-B or BTas-Y plasmid as indicated. A luciferase activity assay was performed 48 h post-transfection. b HEK293T cells (2.5 × 105) were transfected with 100 ng BTas, BTas-B or BTas-Y eukaryotic expression plasmid together with 20 ng pBFV-IP-Luc. Luciferase activity was measured 48 h post-transfection. c Cf2Th cells (8 × 104) were transfected with 200 ng vector, BTas or BTas-B eukaryotic expression plasmid together with 50 ng pBFV-LTR-Luc or pBFV-IP-Luc. Forty-eight hours later, cells were harvested and analyzed using a luciferase activity assay. All results shown are averages from three independent experiments. Error bars indicate SD. *P < 0.05 (unpaired t test). d HEK293T cells (2.5 × 105) were transfected with 200 ng empty vector, BTas-B or BTas-Y eukaryotic expression plasmid together with 800 ng pBS-BFV∆BTas and 50 ng pEGFP-C3. Forty-eight hours later, cells were harvested for Western blotting using indicated antibodies. e Cf2Th cells (1.5 × 105) were transfected with 200 ng empty vector, BTas-B or BTas-Y eukaryotic expression plasmid together with 800 ng pBS-BFV∆BTas. Seventy-two hours later, syncytium was detected in pBS-BFV-B-transfected Cf2Th cells (white arrows)