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Table 2 Primers used in the overlap PCR to introduce a nucleotide mutation to the rescued virus

From: Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30

Primer name

Primer sequencea

Amplification size

PM-1

5′ TCTCCCCATGGTTACTCTGG 3′

839 bp

PM-2

5′ GTCCGTAGAGCCATATAGCAT 3′

PM-3

5′ ATGCTATATGGCTCTACGGAC 3′

657 bp

PM-4

5′ TCTCAGGAGATCTCTCTCCGGACT 3′

  1. aThe restriction enzyme site (NcoI and BglII) in the primers was denoted with italics and the mutated nucleotide set in primers was underlined
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Virology Journal

ISSN: 1743-422X