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Table 1 Primers used for reverse transcription, real-time quantification PCR

From: miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2

Name

Sequence (5’-3’)

TM (oC)

Size (bp)

miR-124-RT

CTCAACTGGTGTCGTGGAGTCGGCAA

  
 

TTCAGTTGAGTGGCATTC

  

miR-124-S

TCGGCAGGTAAGGCACGCGGTG

62

64

miR-124-A

TCAACTGGTGTCGTGGAGTCGGC

  

U6_S

GTGCTCGCTTCGGCAGCACATAT

62

106

U6_A

AAAATATGGAACGCTTCACGAA

  

DNM2- S

CCCACTCGCAAGACCAAA

60

132

DNM2-A

GATGCGATTGATTCGGGC

  

RPL32-S

CGGAAGTTTCTGGTACACAATGTAA

60

96

RPL32-A

TGGAAGAGACGTTGTGAGCAA

  
  1. S Sense primer, A anti-sense primer, RT loop primer for microRNA reverse transcript
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Virology Journal

ISSN: 1743-422X