Fig. 3
From: Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA
Effects of safety treatment on FMDV cDNA by gel electrophoresis and sequencing. a RT-PCR amplified FMDV A23 Iraq P1 coding region observed by gel electrophoresis. Untreated FMDV P1 cDNA in TE pH 8.0 (lane 1), in 250 mM NaCl (lane 2), and safety treated FMDV P1 cDNA (lane 3) are shown. b Sequencing contig assembly chart for alkaline and heat treated FMDV P1 cDNA with sense (green) and antisense (red) sequencing reads shown. c Sequencing contig assembly chart for untreated FMDV P1 cDNA with scale bar. Molecular weight markers are 10 kbp, 4 kbp, 2 kbp, 800 bp and 400 bp (lane M)