Fig. 1
From: Equine rhinitis B viruses in horse fecal samples from the Middle East
a Phylogenetic analysis of nucleotide sequences of the 73-bp fragment (excluding primer sequences) of the partial 5’ UTR of the ERBV detected from four fecal samples of horses in the present study. The four strains with genomes completely sequenced are shaded in gray. The trees were constructed by the neighbor-joining method, and bootstrap values calculated from 1000 trees. Bootstrap values expressed as percentages are shown at nodes and only those >70 % are shown. The scale bar indicates the estimated number of substitutions per 20 nucleotides. Phylogenetic analyses of the (b) P1, (c) VP1, (d) P2, and (e) P3 regions of the four ERBVs (shaded in gray) in the present study and those of other ERBVs. ERAV was included as the outgroup. The scale bars indicate the estimated number of substitutions per 10 (P1), 5 (VP1) and 10 (P2) and 10 (P3) amino acids, respectively. Virus abbreviations (GenBank accession numbers shown in parentheses): AEV, avian encephalomyelitis virus (NC_003990); AiV, Aichi virus A (NC_001918); BHUV1, Hunnivirus A (NC_018668); CPDV-209, Cadicivirus A (JN819202); DHAV-1, duck hepatitis A virus 1 (NC_008250); DPV, avian sapelovirus (NC_006553); EMCV, encephalomyocarditis virus (NC_001479); ERAV, equine rhinitis A virus (NC_003982); ERBV strains, 1576/99 (AY606998), 2225AS (GU799328), 2484c/75 (DQ108385), 263/75 (AY606990), 271AL (GU799329), 271AS (GU799330), 271/75 (AY606991), 293/74 (AY606988), 322/74 (AY606989), 379AL (GU799331), 379/75 (AY606992), 4442/75 (DQ108383), 57-14/89 (AY606994), 58-13/89 (AY606995), 83-11/89 (AY606996), 9051-7/89 (AY606997), KP/92 (AY606993), R4/75 (DQ108384); ERBV1, equine rhinitis B virus 1 (NC_003983); ERBV2, equine rhinitis B virus 2 (AF361253); EV-C, Enterovirus C (NC_002058); FMDV-O, foot-and-mouth disease virus type O (NC_004004); HAV, hepatitis A virus (NC_001489); HCoSV-A1, Cosavirus A (NC_012800); HPeV, human parechovirus (NC_001897); MsPV1, Mischivirus A (JQ814851); MoV, Mosavirus A (NC_023987); PTV-1, porcine teschovirus (NC_003985); RosaV, Rosavirus A (JF973686); SaV, Salivirus A (NC_012957); SePV1, Aquamavirus A (NC_009891); SwPV1, Swine pasivirus 1 (NC_018226); SVV, Seneca Valley virus (NC_011349); TV1, Passerivirus A (NC_014411); TV2, Oscivirus A (NC_014412); TuASV, Avisivirus A (KC465954); TuGV, Gallivirus A (NC_018400); THV-2993D, Megrivirus A (HM751199)