Table 2 Primers used to amplify different regions of the YC2014 genome
Primer | Sequence | Locationa |
|---|---|---|
PEDV1F | ACTTAAAAAGATTTTCTATCTACGG | 1–25 |
PEDV1R | CGTCACTCTCGAAGAATGC | 1986–2004 |
PEDV2F | TCTTTGAGTCTGCCTGTG | 1705–1722 |
PEDV2R | AATTAGCATCACCATCAAATG | 5008–5028 |
PEDV3F | CAGTTTTGCCTAATTTTGAACC | 4729–4750 |
PEDV3R | GAGCCTACGAACTTGTCG | 9011–9028 |
PEDV4F | CGGTGATATGTCTGTTGGC | 8729–8747 |
PEDV4R | TAAATATCAAAATAGCGTTGCAC | 14006–14028 |
PEDV5F | ATCATCACCAGCCCTTGTTG | 13729–13748 |
PEDV5R | TCATTGTCAACTATAATGGCATC | 20006–20028 |
PEDV6F | ACTTCAAGCCAGTGAATGG | 19729–19747 |
PEDV6R | CATCTGGTAGCTGGTCGC | 24332–24349 |
PEDV7F | GCACATTTTCTCTCTGGTAC | 24042–24061 |
PEDV7R | GTGTATCCATATCAACACCG | 28014–28033 |