Fig. 2From: Pre-immune state induced by chicken interferon gamma inhibits the replication of H1N1 human and H9N2 avian influenza viruses in chicken embryo fibroblastsReduced viral titer of H1N1 and H9N2 influenza viruses in CEF expressing chIFN-γ. Twenty-four hours after transfection with pcDNA-IFN-γ, CEFs were infected with H1N1 and H9N2 virus. Forty-eight hours post infection, MDCK cells were treated with a H1N1- and b H9N2-infected CEF culture supernatants. TCID50 titer was measured by end-point dilution assay. Statistical significance was determined by one-way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001)Back to article page