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Fig. 1 | Virology Journal

Fig. 1

From: Pre-immune state induced by chicken interferon gamma inhibits the replication of H1N1 human and H9N2 avian influenza viruses in chicken embryo fibroblasts

Fig. 1

Expression of chIFN-γ in CEFs and the cell culture medium. a CEFs were transfected with pcDNA-IFN-γ. Then, the cell culture medium was harvested at 6, 12, 24, and 48 h post transfection. The medium was centrifuged and concentrated using spin columns. Western blotting was performed as described in Methods. b At 24 h post transfection, the localization of chIFN-γ in CEFs was demonstrated using anti-chIFN-γ polyclonal rabbit antibody as the primary antibody and anti-rabbit IgG Alexa Fluor 488 goat antibody as the secondary antibody (green). Cell nuclei were stained with 4,6-diamidino-2-phenylindole (blue)

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Virology Journal

ISSN: 1743-422X