Fig. 2

Replication renders compact HSV-1 genomes into relaxed structures. All cells were infected with HSV-1 17+ strain for 6 h, then prepared for FISH. In first line, signals are captured from red channel, which were hybridized with Biotin labeled probe; Second line, signals are captured from green channel, which were hybridized with DIG labeled probe; Third line, images are merged to examine the colocalization situation of two color signals; Fourth line, partial enlarged detail of figures in the third line are shown; Fifth line, images from the third line were analyzed, which were done with Image-Pro Plus 6.0 sofrware (USA). a: A brief description of HSV-1 genome structure. Relative to HSV-1 genome, red probe labeled with Biotin locates at the right terminal, which contains IR_S, TR_S and U_S region (according to NC_001806.2 127235–131131, 132647–133909, 134056–134931, 135225–136670, 136747–137463, 138423–139607, 139789–140961, 141247–142899 and 147066–150962). Green probe labeled with DIG locates at the right terminal, which contains TR_L, IR_L and partial U_L region (according to NC_001806.2, 513–1259, 2262–2318, 3084–3750, 3887–5490, 9338–10012, 10991–11665, 12484–15132, 151131–17161, 18225–20477, 20705–23260, 120884–122487, 122624–123290, 124056–124112 and 125115–125861). b-g: Cells were infected at a MOI of 0.1 PFU/cell. At early stage of HSV-1 replication, images are captured with STED microscopy and then analyzed. h-m: Cells were infected at a MOI of 5 PFU/cell. At late stage of HSV-1 replication, images are captured with STED microscopy and then analyzed. Host cell nucleus are indicated with white dotted lines. e, f, k, l: Higher zooms of regions inside the white rectangles are shown. Scale bars, 2.5 μm. Rr: correlation coefficient; R: overlapping coefficient; k: antigen contribution