Fig. 2
From: S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen
Expression of the recombinant PEDV S1 protein in insect Sf9 cells. a A small scale S1 protein expression experiment was performed by infecting Sf9 cells with recombinant baculovirus expressing PEDV S1 gene. Cell culture media were collected on indicated days post-infection and analysed by Western blotting using anti-his antibody. b Large scale S1 protein purification was performed on cell culture medium of the cells infected with recombinant baculovirus at day 7 post-infection, and the purified protein was anyalzed by SDS-PAGE and stained by Coomassie Blue. Medium from un-infected cells served as a negative control. Numbers on the left indicate the protein molecular marker size in kDa