Fig. 1
From: S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen
Expression of the recombinant PEDV S1 protein in PichiaPink Pichia pastoris. a A small scale S1 protein expression experiment was performed, and samples of culture medium were collected on indicated days post-induction and detected by Western blotting using anti-his antibody. b A large scale S1 protein purification was performed from the yeast cell supernatant at day 4 post-induction, and the purified protein was anyalzed by staining of SDS-PAGE by silver nitrate. Medium from the un-induced cells served as a negative control. Numbers on the left indicate the protein molecular marker size in kDa