Fig. 2From: Development of an HSV-1 neutralization test with a glycoprotein D specific antibody for measurement of neutralizing antibody titer in human seraOptimized detection for HSV-1. a Infection kinetics of HSV-1. The number of spots during HSV-1 infection at a MOI of 0.005 was monitored for 108 h. The average numbers of spots were calculated from triplicate experiments, and the error bars show the standard deviations. b Spot detection with different dilutions of 2G5 MAb. The monolayer U-2 OS cells were infected with KOS at a MOI of 1 × 104 PFU, then detected by serially diluted 2G5 detection antibody. c Relationship between the infectious dose and the number of spots. The number of spots was obtained from triplicate infection under a series of infectious doses ranged from 2000 PFU to 12000 PFU. An irrelevant antibody was used as a negative controlBack to article page