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Fig. 2 | Virology Journal

Fig. 2

From: Development of an HSV-1 neutralization test with a glycoprotein D specific antibody for measurement of neutralizing antibody titer in human sera

Fig. 2

Optimized detection for HSV-1. a Infection kinetics of HSV-1. The number of spots during HSV-1 infection at a MOI of 0.005 was monitored for 108 h. The average numbers of spots were calculated from triplicate experiments, and the error bars show the standard deviations. b Spot detection with different dilutions of 2G5 MAb. The monolayer U-2 OS cells were infected with KOS at a MOI of 1 × 104 PFU, then detected by serially diluted 2G5 detection antibody. c Relationship between the infectious dose and the number of spots. The number of spots was obtained from triplicate infection under a series of infectious doses ranged from 2000 PFU to 12000 PFU. An irrelevant antibody was used as a negative control

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