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Fig. 1 | Virology Journal

Fig. 1

From: Development of an HSV-1 neutralization test with a glycoprotein D specific antibody for measurement of neutralizing antibody titer in human sera

Fig. 1

Specificity of MAbs in detecting the HSV-1 infected cells. a 12 MAbs were selected from a panel of 60 antibodies developed in our laboratory for specifically recognizing the HSV-1 infected cells. The Cell based ELISPOT assay was used in the detection. b Schematic image of the 2G5 antibody in detecting the HSV-1 (KOS strain) infected cells and mock-infected cells, the shown blue spots indicate the viral infection. c The reactivity of 2G5 against other HSV viruses. U-2 OS cells were infected with HSV-2 (Strain 186), HSV-1 (Strain 16, Strain F, Strain KOS), cells were harvested 48 h after transfection and total protein was extracted and analyzed by Western Blot. d The reactivity of 2G5 against HSV-1 glycoproteins. U-2 OS cells were transfected with 12 glycoprotein constructs (plv-gB ~ gN). Cells were harvested 48 h after transfection and total protein was extracted and analyzed by Western Blot. KOS infected cell lysate was taken as a control. e Immunofluorescence analysis of the specificity of 2G5 MAb. After plv-gD transfection and puromycin selection, gD expressing U-2 OS cells were established and stained with 2G5 MAb. U-2 OS mock cells transfected with empty lentivirus vector and selected with puromycin were analyzed as a control

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