Fig. 1

Cell entry and subsequent virus gene transcription of RSV in monocytes is actin-dependent. a Gating strategy to determine the amount of internalization of RSV in monocytes. b Monocytes were pre-treated with RPMI, CytoD (1 μg/ml), Wisko (10 μM) or CPZ (5 μg/ml). After pre-treatment, monocytes were incubated with RPMI or RSV at MOI 1 for 1 h at 37 °C and internalization was measured. c Monocytes were pre-treated with RPMI, CytoD (1 μg/ml), or CPZ (5 μg/ml). After pre-treatment, monocytes were incubated with RPMI or RSV at MOI 1 for 1 h at 4 °C and binding was measured. d PBMCs were pre-treated with RPMI, CytoD (1 μg/ml) or CPZ (5 μg/ml). After pre-treatment, PBMCs were incubated with RPMI or RSV at MOI 1 for 24 h and virus gene transcription was measured in monocytes. One-way analysis of variance with Bonferroni’s Comparison Test was used for statistical analysis. Data are normalized to untreated cells and depicted as mean ± SEM (N = 4-6). (**p < 0.01, ***p < 0.001) (NS = no significant difference between untreated and CytoD-treated cells or between untreated and CPZ-treated cells)