Table 1 Primer sequences for quantitative real-time polymerase chain reaction
RNA target | Forward primer (5' → 3') | Reverse primer (5' → 3') | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
Chicken | ||||
 GAPDH | CCTCTCTGGCAAAGTCCAAG | CATCTGCCCATTTGATGTTG | 200 | NM_204305 |
 TLR3 | ACAATGGCAGATTGTAGTCACCT | GCACAATCCTGGTTTCAGTTTAG | 123 | NM_001011691 |
 TLR7 | TGTGATGTGGAAGCCTTTGA | ATTATCTTTGGGCCCCAGTC | 218 | DQ780342 |
 IL-1β | GCTCTACATGTCGTGTGTGATGAG | TGTCGATGTCCCGCATGA | 80 | NM204524 |
 IL-6 | CCTGTTCGCCTTTCAGACCT | GGGATGACCACTTCATCGGG | 171 | EU170468 |
 IL-8 | ATTCAAGATGTGAAGCTGAC | AGGATCTGCAATTAACATGAGG | 196 | DQ393272 |
 LITAF | CCGCCCAGTTCAGATGAGTT | GCAACAACCAGCTATGCACC | 130 | AY765397 |
 IFN-a | ATGCCACCTTCTCTCACGAC | AGGCGCTGTAATCGTTGTCT | 387 | EU367971 |
 IFN-r | TGAGCCAGATTGTTTCGATG | CTTGGCCAGGTCCATGATA | 248 | DQ906156 |
 MHC-I | AAGAAGGGGAAGGGCTACAA | AAGCAGTGCAGGCAAAGAAT | 222 | NM001031338 |
 MHC-II | CTCGAGGTCATGATCAGCAA | TGTAAACGTCTCCCCTTTGG | 312 | DQ008588 |
Duck | ||||
 GAPDH | ATGTTCGTGATGGGTGTGAA | CTGTCTTCGTGTGTGGCTGT | 176 | AY436595 |
 TLR3 | GAGTTTCACACAGGATGTTTAC | GTGAGATTTGTTCCTTGCAG | 200 | NM_001310782 |
 TLR7 | CCTTTCCCAGAGAGCATTCA | TCAAGAAATATCAAGATAATCACATCA | 154 | AY940195 |
 IL-1β | TCGACATCAACCAGAAGTGC | GAGCTTGTAGCCCTTGATGC | 185 | DQ393268 |
 IL-6 | TTCGACGAGGAGAAATGCTT | CCTTATCGTCGTTGCCAGAT | 150 | AB191038 |
 IL-8 | AAGTTCATCCACCCTAAATC | GCATCAGAATTGAGCTGAGC | 174 | AB236334 |
 LITAF | ACAGGACAGCCTATGCCAAC | CATCTGAACTGGGCGGTCAT | 96 | EU375296 |
 IFN-a | TCCTCCAACACCTCTTCGAC | GGGCTGTAGGTGTGGTTCTG | 232 | EF053034 |
 IFN-r | GCTGATGGCAATCCTGTTTT | GGATTTTCAAGCCAGTCAGC | 247 | AJ012254 |
 MHC-I | GAAGGAAGAGACTTCATTGCCTTGG | CTCTCCTCTCCAGTACGTCCTTCC | 196 | AB115246 |
 MHC-II | CCACCTTTACCAGCTTCGAG | CCGTTCTTCATCCAGGTGAT | 229 | AY905539 |
 Chicken and Duck IFN-β | CCTCAACCAGATCCAGCATT | GGATGAGGCTGTGAGAGGAG | 259 | AY831397 |