Fig. 2
From: Inhibition of protein kinase C promotes dengue virus replication
Inhibitions of PKC by PKCα siRNA or a chemical inhibitor (BisI) suppressed NS5 RdRp phosphorylations. a PKCα-specific siRNA depleted endogenous PKCα at 24, 48, 72 h after siRNA transfection. A pool of PKCα siRNAs (siPKCα) was transfected into the BHK-21 cells. The levels of PKCα were detected by PKCα immunoblotting. A non-targeting siRNA (siNeg) was used as a control. GAPDH immunoblots were used as a loading control. b PKCα depletion by siRNA downregulated NS5 Threonine and Serine phosphorylations. BHK-21 cells were infected with recombinant DENV expressing His-FLAG-tagged NS5. The His-FLAG-tagged NS5 was pulled down (IP) from BHK-21 cell lysate by anti-FLAG antibody, and Threonine/Serine phosphorylations (p-Thr/p-Ser) were examined by p-Thr or p-Ser-specific antibodies. c Quantifications of p-Thr/NS5 and p-Ser/NS5 from (b)