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Table 2 Putative recombination events in the genomes and individual open reading frames of viruses of RMV cluster of subgroup 3 tobamoviruses

From: Molecular characterisation of a novel recombinant Ribgrass mosaic virus strain FSHS

   

Parental isolate

Break points

 
 

Method

Recombinant

Major

Minor

Beginning

Ending

Average P-value

Genome

RDP

RMV FSHS

RMV NZ-439

RMV Actinidia-AC

1906

2564

5×10-26

 

GeneConv

2148

2479

5×10-10

 

Bootscan

1905

2564

8×10-23 − 5×10-22

 

MaxChi

1906

2564

3×10-17 − 8×10-14

 

Chimaera

1926

2558

1×10-06 − 3×10-05

 

SciScan

2148

2414

1×10-10 − 6×10-10

 

3Seq

1926

2558

2×10-21 − 5×10-06

ORF 1-2

RDP

RMV FSHS

RMV NZ-439

RMV Actinidia-AC

1839−1858

2498

5×10-23 − 9×10-20

 

GeneConv

1839

2498

8×10-11 − 3×10-08

 

Bootscan

1839

2498

3×10-22 − 2×10-18

 

MaxChi

1839

2498

6×10-18 − 3×10-12

 

Chimaera

1839

2498

2×10-16 − 1×10-02

 

SciScan

1807−1862

2461−2522

3×10-11 − 4×10-10

 

3Seq

1839

2498

2×10-22 − 8×10-04

ORF 3

 

No recombination

ORF 4

 

No recombination

  1. RDP, Bootscan and SiScan are phylogeny-based methods, while GeneCov, MaxChi, and Chimaera are substitution-based methods. 3SEQ recombination detection algorithm tests for mosaicism between three nucleotide sequences. No recombinations were detected using Lard. ‘Major’ and ‘Minor’ parents refer to parental isolates contributing the larger and smaller fractions of recombinant sequence respectively. P-values < 0.05 were considered significant. [Genomes of RMV strains: Kons. 1105-V2 isolate R14 (HQ667980) and Actinidia-AD (GQ401366.1) were not included in the analysis as they were 99 % and 100 % similar to RMV Kons. 1105 isolate R14 (HQ667979) and Actinidia-AC (GQ401365.1) respectively]