Fig. 3

Colocalization of GCRV-JX01 and clathrin in endosomes. a Purified intact GCRV virions. Magnification, ×30,000 (panels A), Scale bars represent 50 nm and 100 nm. b Western blot analysis of purified GCRV particles and clathrin in CIK cells with polyclonal antibody against VP5 or anti-Clathrin-heavy chain antibody. c CIK cells were incubated with GCRV-JX01 virions (MOI = 50) on ice for 30 min, and then transferred to 28 °C. Cells were washed, fixed and analyzed by IFA with anti-GCRV JX01 VP5 antibody(green) plus anti-Clathrin-heavy chain antibody(red). Scale bars represent, 10 μM