Fig. 2

Effects of inhibitors on GCRV infection. a Virus yield in CIK cells pretreated with vehicle, 10 mM NH₄Cl, 20 μM CQ, 50 μM CPZ, or 100 μM of dynasore. b Western blotting analysis to monitor viral replication level. Cells were treated with various inhibitors as described above. After 12 h of incubation, cells were lysed and processed for Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with vehicle. c Virus yield in CIK cells pretreated with vehicle, 5 μg/mL Filipin III, 50 μM LY294002, or 25 μg/mL nystatin. d Western blotting analysis to monitor the viral replication level. Cells were treated with inhibitors as described above. After 12 h of incubation, cells were lysed and processed for Western blot analysis of GCRV-VP5 protein expression with polyclonal mouse serum. GAPDH was included as an internal loading control for the Western blots. NC denotes the negative control treated with vehicle. Each experimental point is the sum of triplicate experiments with two titer assays. Error bars represent the standard errors of the mean. Asterisks represent a significant difference from the control (un-paired t-test)