Fig. 8

Trypan blue staining is detected in the HEp2 cell monolayers. HEp2 cell monolayers were either mock-infected or RSV-infected using an multiplicity of infection of 0.0002 and (a-f) at between 1 and 5 days post-infection (dpi) the monolayers were stained using trypan blue and imaged using an inverted light microscope (objective x10 magnification). The regions of the cell monolayer showing trypan blue staining is highlighted (black arrows). In plate (c) selected regions in the monolayer (indicated by open white box) are enlarged in the inset. Plate (e) shows enlarged images from the region indicated in plate (d) (indicated by open white box)