Fig. 3

Viral morphogenesis in K-cESCs infected with vBAC20UL17mRFP. a K-cES-KP2 infected cell. b Enlargement of a part of the nucleus of K-cES-KP2 infected cell showing typical A, B and C capsids. c Enlargement of the lower boxed area on picture A showing cisternae of the nuclear membrane containing 2 primary enveloped viral particles and a granular material embedded in a single membrane (black square) Primary enveloped particles in nuclear membrane cisternae are often accompanied by single membrane embedded material, as if the primary envelopment of viral capsids was concomitant to the embedding of cellular material; 2 primary enveloped particles in a nuclear vacuole are present on the right. d Enlargement of the area boxed in grey in the cytoplasm of K-cES-KP2 cell on picture A. Numerous C capsids in the cytoplasm are close to a cytoplasmic vacuole containing primary enveloped particles. e Another K-cES-KP2 infected cell showing at least 11 viral particles in a cisternae close to the nuclear membrane (black circle). Note the abundant network of transversally sectioned fibres in the cytoplasm (KF). f Enveloped mature particle in a K-cES-K1 infected cell (black arrow). N : Nucleus; C : Cytoplasm; Black Stars : C capsids; Black triangle : A capsids; White triangle : B capsids; Black dashed circle : primary enveloped particles; White circle : degenerating lamellar bodies; KF : Keratin filaments