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Table 2 The plaque numbers of serum samples in immunized mice by plaque reduction neutralization test (PRNT) assay

From: Construction and immune efficacy of recombinant pseudorabies virus expressing PrM-E proteins of Japanese encephalitis virus genotype І

Dilution of Seruma Numbers of Plaque by PRNT
DMEM PRV TK/gE/LacZ+ PRV TK/gE/PrM-E+ JEV Vaccine
1:4 64.5 ± 0.5 59.5 ± 1.5 23.5 ± 1.5 11.0 ± 1.0
1:8 62.5 ± 2.5 57.5 ± 2.5 35.0 ± 1.0 19.5 ± 0.5
1:16 63.5 ± 1.5 60.5 ± 0.5 55.5 ± 0.5 24.0 ± 1.0
1:32 62.5 ± 0.5 63.5 ± 1.5 60.5 ± 1.5 34.0 ± 1.5
1:64 63.0 ± 1.5 62.5 ± 0.5 63.5 ± 0.5 62.5 ± 2.5
  1. aThe sera of mice at 8 weeks post immunization was diluted and mixted with 200PFU of JEV SX/09/01 strain for 1 h at 37 °C, and then added to BHK-21 in 24-well plate by PRNT assay