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Table 2 The plaque numbers of serum samples in immunized mice by plaque reduction neutralization test (PRNT) assay

From: Construction and immune efficacy of recombinant pseudorabies virus expressing PrM-E proteins of Japanese encephalitis virus genotype І

Dilution of Seruma

Numbers of Plaque by PRNT

DMEM

PRV TK−/gE−/LacZ+

PRV TK−/gE−/PrM-E+

JEV Vaccine

1:4

64.5 ± 0.5

59.5 ± 1.5

23.5 ± 1.5

11.0 ± 1.0

1:8

62.5 ± 2.5

57.5 ± 2.5

35.0 ± 1.0

19.5 ± 0.5

1:16

63.5 ± 1.5

60.5 ± 0.5

55.5 ± 0.5

24.0 ± 1.0

1:32

62.5 ± 0.5

63.5 ± 1.5

60.5 ± 1.5

34.0 ± 1.5

1:64

63.0 ± 1.5

62.5 ± 0.5

63.5 ± 0.5

62.5 ± 2.5

  1. aThe sera of mice at 8 weeks post immunization was diluted and mixted with 200PFU of JEV SX/09/01 strain for 1 h at 37 °C, and then added to BHK-21 in 24-well plate by PRNT assay
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Virology Journal

ISSN: 1743-422X