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Table 3 Comparison of ORFV exo RPA assay with qPCR assay on clinical samplesa

From: Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus

   qPCR
   Positive Negative  
RPA Positive 14 0 14
  Negative 2 19 21
   16 19 35
  1. aSamples include twenty two samples collected from suspected cases of the orf, eight nasal swabs collected from experimentally infected sheep and five samples obtained from healthy goats. All samples either ORFV or no viral DNAs detected