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Table 1 RPA primers and probes designed in this study

From: Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus

Name

Sequence (5’ –3’)

Genome location(U33419.1)

ORFVRPA F1

CTAGTAAGCTGTTCGAGATCACCTTGTTCATCATG

4465-4499

ORFV RPA F2

TTCCGACGGACGTATGAATATGTCCATGGTGAACG

4530-4564

ORFV RPA F3

CGTATGAATATGTCCATGGTGAACGATGTACCAAC

4540-4574

ORFV RPA R1

AGCGTTCATTCAATTCATGTCTGAGGTAAACGGCA

4702-4736

ORFV RPA R2

GACCACGTAAAAGTGGTGTTCGAAAAACTTCACAA

4651-4685

ORFV RPA R3

GGTAAACGGCAATGATGTTCGTGACAAAGACCACG

4679-4713

ORFV RPA P

AACGTATCCCATGCAGTAAAGCATAGTCCG

4582-4631

 

(FAM-dT)C(THF)C(BHQ1-dT)TATAAACTCAGGAAC-p

 
  1. ORFV RPA F and R, RPA primer; ORFV RPA P, RPA Exo probe; BHQ1-dT, thymidine nucleotide carrying Black Hole Quencher 1; THF, tetrahydrofuran spacer; FAM-dT, thymidine nucleotide carrying fluorescein; P (phosphate), block elongation
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Virology Journal

ISSN: 1743-422X