Fig. 2
From: Nonstructural protein Pns4 of rice dwarf virus is essential for viral infection in its insect vector
Microinjection of dsPns4 inhibited RDV infection and spread in insect vectors in vivo. At 12 days padp, the dissected intestines a and b and salivary glands (c and d) from leafhoppers receiving dsGFP or dsPns4 were immunolabeled with virus-FITC (green) (a and c), Pns4-FITC (green) (b and d) or Pns12-rodanmine (red) (b and d). The images with green fluorescence or red fluorescence were merged under a background of transmitted light. The enlarged images showing green fluorescence (Pns4-FITC) and red fluorescence (Pns12-rodanmine) of the merged images in the boxed areas in each panel, indicated that both of minitubules and diffusion of Pns4 distributed at the edge of viroplasms. fc, filter chamber; amg, anterior midgut; mmg, middle midgut; pmg, posterior midgut; hg, hindgut; mt, Malpighian tubules; sg, salivary gland. Bars, 100 μm (a and c) and 10 μm (b and d)