Fig. 6

Co-expression of VP2 with VP6 and VP6/8*(N/C). a Western blot analysis of crude extract of plants co-expressed with VP2 + VP6/8*. b Western blot of crude extract of plants co-expressed with VP2 + VP6. VP2 appears to be in higher amounts when co-expressed with VP6. Proteins were detected using anti-VP6 antibody (1/5000) and anti-histidine (1/2000). M – Pre-stained molecular marker, −ve – plants with infiltration media only. N – VP8* epitope fused to the amino terminus of VP6. C – VP8* epitope fused to the carboxy terminus of VP6. Arrows indicate expected band size (VP6*/8(N/C) ± 43 kDa, VP2 ± 100 kDa, VP6 ± 42 kDa)