Fig. 3
From: Herpes simplex virus 1 UL41 protein abrogates the antiviral activity of hZAP by degrading its mRNA
HSV-1 UL41 protein promotes hZAP mRNA degradation. a 293Trex-hZAPL or 293Trex-hZAPS cells were transfected with pCMV-Flag control vector or increasing amount of UL41-Flag plasmid. At 6 h post-transfection, cells were mock treated or treated with tetracycline (1000 ng/mL) to induce hZAPL or hZAPS expression. Quantitative RT-PCR analysis was then performed to detect the mRNA level of hZAPL or hZAPS. b 293Trex-hZAPL cells or 293Trex-hZAPS cells were infected with WT HSV-1 or R2621 at an MOI of 1 or 10, respectively. At 2 h post-infection, cells were mock treated or treated with tetracycline (1000 ng/mL). Quantitative RT-PCR analysis was then performed to detect the mRNA level of hZAPL or hZAPS. One representative of three independent experiments was shown. (*P < 0.05, **P < 0.01)