Fig. 1

Subtype B and C Nef sequences and sites of mutation. The first 100 codons of the subtype B Nef sequences (NL4-3 and B47) and the subtype C Nef sequence (SK68) into which mutations were introduced are shown relative to the consensus B (Con_B) and consensus C (Con_C) Nef sequences. Sequences were aligned to HXB2 (and the subtype C specific insertion in the ₆₂EEEE₆₅ motif with respect to HXB2 was stripped out). Codons at which mutations were introduced are shown in bold. It should be noted that, prior to commencing experiments, the NL4-3 Nef sequence was mutated to the subtype B consensus residue valine at codon 11 since the wild-type contains isoleucine