Fig. 1

SRSF1 mediated suppression of JCV-early and -late gene transcription is complemented by T-Antigen. a. Luciferase assay showing the effects on transcriptional activity of the JCV early promoter region by SRSF1, T-antigen, and agnoprotein. PHFA cells were co-transfected with pLuc.JCV-Early and the listed expression plasmids. At 48 h post transfections, cell lysates were prepared and analyzed by luciferase reporter assay. Luminescence was determined and normalized to protein concentrations. Standard deviations were calculated from three independent experiments are presented as bar graph. Orientation of JCV Mad1 early promoter (GenBank: J02226.1) and firefly luciferase are schematized and shown in the upper panel of the bar graph. b. Western blot analysis of whole cell extracts prepared in parallel to the samples in panel A.SRSF1 encoded by plasmid with a T7 tag is distinguished from endogenous SRSF1 levels. c. PHFA cells were transiently transfected with pLuc.JCV-Late and listed plasmid combinations. At 48 h post transfections, cell lysates were prepared and analyzed by luciferase reporter assay. Luminescence was determined and normalized to protein concentrations. Standard deviations were calculated from three independent experiments are presented as bar graph. The orientation of JCV Mad1 late promoter (GenBank: J02226.1) and firefly luciferase are schematized and shown in the upper panel of the bar graph. d. Western blot analysis of whole cell extracts prepared in parallel to the samples in panel c