Fig. 4
From: Evidence that HIV-1 restriction factor SAMHD1 facilitates differentiation of myeloid THP-1 cells
Reduction of SAMHD1 expression after T cell activation. Total peripheral blood CD3+ or CD4+ T cells (extracted from peripheral blood mononuclear cells with BD Bioscience CD3+ or CD4+ negative-selection kit, respectively) were activated by incubation with CD3/CD28 beads (1bead/5cells). Total SAMHD1 levels were assessed in whole cell extracts by western-blot at 0 (T0), 4 (T1) 8 (T2), 24 (T3), 36 (T4), 48 (T5), 72 (T6) hours after activation (top) and normalized to GAPDH for quantification (bottom). One representative experiment among three is shown