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Fig. 4 | Virology Journal

Fig. 4

From: Polyethylene glycol-mediated fusion of herpes simplex type 1 virions with the plasma membrane of cells that support endocytic entry

Fig. 4

Detection of HSV-1 replication following PEG fusion. At 6 or 24 h following PEG treatment of HSV-1 bound to (a) B78 or (b) CHO cells (MOI of 10), DNA was extracted from cells with PureGene Kit (Qiagen) or from supernatant (Extracellular) with QIAamp DNA Blood Mini Kit (Qiagen). A standard curve for the assay was generated using known numbers of copies of a plasmid containing the HSV-1 ICP22 coding region diluted in glycogen. (c) Permissive control B78-nectin-1 cells were infected with HSV-1 for 24 h and processed as in panels a and b. 2.18E + 03 HSV-1 DNA genome copies were detected in the extracellular sample

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Virology Journal

ISSN: 1743-422X