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Fig. 3 | Virology Journal

Fig. 3

From: Polyethylene glycol-mediated fusion of herpes simplex type 1 virions with the plasma membrane of cells that support endocytic entry

Fig. 3

PEG-mediated entry leads to HSV-1 gene expression. At 6 or 24 h following either PEG treatment of HSV-1 bound to (a) B78 or (b) CHO cells or (c) following HSV-1 infection of B78-nectin-1 cells, RNA was extracted with TRIzol (Ambion). Following DNase treatment, first-strand cDNA was synthesized from 1.75 micrograms of total RNA using SuperScript VILO (Invitrogen). Equal volumes of cDNA were used to quantify HSV-1 gene ICP27, thymidine kinase (TK) and gC transcripts with Sso Advanced Sybr Green SuperMix (Bio-Rad), using a Bio-Rad CFX96 Real Time System. The quantity of HSV-1 mRNAs, normalized to cellular GAPDH, is shown relative to mRNA from 6 h post-PEG fusion

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Virology Journal

ISSN: 1743-422X