Fig. 2

Quality control of the screen. a Screen plate design. A representative 384-well plate is shown to illustrate the locations of sample and control siRNAs. Sample siRNAs (red) are arranged in column 1 through column 22. Non-targeting siRNAs (blue) and siRNAs targeting ATP6V0C (green) or luciferase (yellow) are arranged in columns 23 and 24 respectively. b Signal distribution of samples and controls. The sample and control luciferase signals are normalized by the median signal value of all the samples in each 384-well assay plate. The normalized signal distributions in both Marburg virus (MARV) and influenza H5N1 virus (AIV) plates are plotted for sample (red) and controls: ATP6V0C (green), luciferase (yellow) and non-targeting (blue). c Z’ factors. Z’ factors are computed either by the normalized signal values of luciferase and non-targeting controls (luc vs nt) or those of ATP6V0C and non-targeting controls (atp6 vs nt). These Z’ factors are plotted for both MARV and AIV plates