Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR

Fang, Peng; Lu, Rongfei; Sun, Feng; Lan, Ying; Shen, Wenbiao; Du, Linlin; Zhou, Yijun; Zhou, Tong

doi:10.1186/s12985-015-0405-2

Virology Journal

Table 1 Candidate reference genes and their primer sequences used in this study

From: Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR

Gene name	Accession number	Gene description	Primer sequence (5′ → 3′)	Amplicon length (bp)
Actin	AK058421	Actin	F-CAGCCACACTGTCCCCATCTA	86
Actin	AK058421	Actin	R-AGCAAGGTCGAGACGAAGGA	86
UBC	AK059694	Ubiquitin-conjugating enzyme E2	F-CCGTTTGTAGAGCCATAATTGCA	76
UBC	AK059694	Ubiquitin-conjugating enzyme E2	R-AGGTTGCCTGAGTCACAGTTAAGTG	76
18S rRNA	AK059783	18S ribosomal RNA	F-CTACGTCCCTGCCCTTTGTACA	65
18S rRNA	AK059783	18S ribosomal RNA	R-ACACTTCACCGGACCATTCAA	65
EF-1α	AK061464	Eukaryotic elongation factor1-alpha	F-TTTCACTCTTGGTGTGAAGCAGAT	103
EF-1α	AK061464	Eukaryotic elongation factor1-alpha	R-GACTTCCTTCACGATTTCATCGTAA	103
UBQ 5	AK061988	Ubiquitin 5	F-ACCACTTCGACCGCCACTACT	69
UBQ 5	AK061988	Ubiquitin 5	R-ACGCCTAAGCCTGCTGGTT	69
GAPDH	AK064164	Glyceraldehyde-3-Phosphate dehydrogenase	F-AAGCCAGCATCCTATGATCAGATT	79
GAPDH	AK064164	Glyceraldehyde-3-Phosphate dehydrogenase	R-CGTAACCCAGAATACCCTTGAGTTT	79
α-TUB	AK067721	Alpha-tubulin	F-GGAAATACATGGCTTGCTGCTT	89
α-TUB	AK067721	Alpha-tubulin	R-TCTCTTCGTCTTGATGGTTGCA	89
β-TUB	AK072502	Beta-tubulin	F-GCTGACCACACCTAGCTTTGG	82
β-TUB	AK072502	Beta-tubulin	R-AGGGAACCTTAGGCAGCATGT	82
eIF-4α	AK073620	Eukaryotic-initiation factor 4α	F-TTGTGCTGGATGAAGCTGATG	76
eIF-4α	AK073620	Eukaryotic-initiation factor 4α	R-GGAAGGAGCTGGAAGATATCATAGA	76
Actin1	AK100267	Actin1	F-CTCCCCCATGCTATCCTTCG	67
Actin1	AK100267	Actin1	R-TGAATGAGTAACCACGCTCCG	67
UBQ 10	AK101547	Ubiquitin 10	F-TGGTCAGTAATCAGCCAGTTTGG	65
UBQ 10	AK101547	Ubiquitin 10	R-GCACCACAAATACTTGACGAACAG	65
TIP41-Like	AK103511	TIP41-like family protein	F-GTTTGGATGAACCCCGCAA	75
TIP41-Like	AK103511	TIP41-like family protein	R-GGCAACAAGGTCAATCCGATC	75
EXP	Os06g11070	Expressed protein	F-AGGCTGGTCGAGGAGTCCAT	84
EXP	Os06g11070	Expressed protein	R-TTCTCCTCCCTAGCGAACACCT	84
Os AOC	AI493664	Allene oxide cyclase	F-CCACCATCACAGATCGGATCTT	78
Os AOC	AI493664	Allene oxide cyclase	R-GCGGTCAGAGCGAAAGTAGCTA	78

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ISSN: 1743-422X

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