Fig. 3
From: Bombyx mori nucleopolyhedrovirus (BmNPV) Bm64 is required for BV production and per os infection
a Virus growth curves determined by the TCID50 endpoint dilution assays. BmN cells were infected at an MOI of 3 for each virus, and the supernatants were harvested at the selected time points for the titer assay. Each data point was determined from the average of three independent infections; the error bars represent the standard deviations. b BV production independent of virion infectivity was determined by quantifying the number of viral genomes by real-time PCR analysis of the supernatants harvested from each bacmid-transfected cell at the designated time points. Each value represents the average of three independent transfections, and the error bars indicate the standard deviations. c Real-time PCR analysis of viral DNA replication. BmN cells were transfected with vBm, vBm64KO or vBm64RE. At the designated time points, total intracellular DNA was extracted, digested with the restriction enzyme Dpn I to eliminate input bacmid DNA, and analyzed by real-time PCR. The graph shows the results of three independent replication assays, with error bars indicating the standard deviations