Fig. 2
From: Prevalence of human parvovirus B19 in Chinese plasma pools for manufacturing plasma derivatives
Standard curve of the real-time qPCR assay for B19V DNA. a Amplification plots showing the testing in duplicate of a 10-fold dilution series containing standard plasmid of B19V from 1 × 108 to 1 × 101 template copies per μL, and the IC plasmid at 120 copies per reaction. b Real-time PCR standard curve generated from plasmid DNA amplification plots(a). The x-axis represents B19V standard plasmid in 10-fold dilutions and the y-axis represents the fluorescence data used for Cq determinations. The assay was linear in the range of 1 × 108 to 1 × 101 template copies per μl, with an R2 of 1.000, the slope value of −3.299 and reaction efficiencies of 101.0 %