Fig. 4

DENV-2 induces the expression of IFN-III in C33-A cells. C33-A cells were treated with IFN-λ1 (35 ng/ml) infected with DENV-2 (MOI = 0.1) or pre-treated with IFN-λ1 and subsequently infected with DENV-2. Forty-eight h post-infection, total cellular RNA was isolated and used in end-point RT-PCR for qualitative determination of the IFN-λ1, IFN-λ2, IFN-λ3 genes, using HPRT as an endogenous control gene. a Results of RT-PCR amplification of mock-infected and the three experimental conditions are shown. b Extracts of total protein from each condition were used for Western blotting to determine the presence of IFN-λ1 and IFN-λ2 proteins. Actin was the loading control. A semi quantitative analysis of detected bands normalized to actin was performed using ImageJ software (http://rsb.info.nih.gov/ij/)