Fig. 4

A20/ABIN1 overexpression contributes to abnormal M1 polarization. A20 and ABIN1 were over expressed in THP-1 cells (incubated with LPS/IFN-γ before analysis) during PMA stimulation. a A20/ABIN1 over-expression reduced CD100 and TfR1 expression and CD163 was slightly up regulated. b A20/ABIN1 over-expression influences MMPs expression differently. c IL-10 and IL-12 were decreased by A20/ABIN1 over-expression. d Over-expression of A20 or ABIN1 inhibited NF-κB activity, and co-expression had a greater efficacy (upper). NF-κB activity was gradually reduced at 3 days incubation (lower)