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Table 1 Total RNA isolated from old leaves of grapevines with Sigma kit using the standard or modified protocol

From: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

Leaf samples

RNA yield (μg)

A260/280

A260/230

 

Standard

Modified

Standard

Modified

Standard

Modified

CF-1

3.1

8.8

1.08

2.08

0.29

2.00

CF-2

1.2

8.5

1.49

2.06

0.48

2.03

CF-3

2.1

8.6

1.69

2.06

0.64

2.06

CF-4

1.0

5.7

0.71

2.14

0.11

1.56

CF-5

0.5

5.9

0.90

2.06

0.13

1.90

CF-6

0.6

13.1

1.08

2.03

0.21

2.11

CF-7

1.8

8.1

0.86

2.03

0.18

1.95

CF-8

4.8

9.5

0.94

1.99

0.57

1.85

CF-9

4.6

12.4

0.95

1.99

0.38

2.03

CF-10

1.4

13.0

0.78

2.10

0.16

1.94

CF-11

4.8

16.8

0.81

1.87

0.42

2.17

Average

2.4

10.0

1.03

2.04

0.32

1.96

  1. 50 mg of old and symptomatic leaves of Vitis vinifera var. Cabernet Franc was used in RNA isolation using Spectrum™ Plant Total RNA Kit (Sigma) with standard protocol (Standard) or modified protocol with 2.5 % of PVP-40 being added in the lysis solution (Modified). The total RNA yield (μg), A260/A280 and A260/A230 ratios were determined with a NanoDrop spectrophotometer
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Virology Journal

ISSN: 1743-422X