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Table 1 Effect of mutations introduced into the MVEV octapeptide and at the P1’ residue on NS1-2A cleavage

From: Proteolytic cleavage analysis at the Murray Valley encephalitis virus NS1-2A junction

P8 P7 P6 P5 P4 P3 P2 P1 P1’ % cleavagea (mean ± SEM)
L V K S R V Q A F 88 ± 1
         Y 84 ± 0
         V 80 ± 5
         G 86 ± 3
        S   53 ± 2
        L   6 ± 1
        F   7 ± 0
        R   7 ± 0
        D   10 ± 1
       A    88 ± 2
       Y    79 ± 3
       G    37 ± 4
       D    89 ± 4
       L    89 ± 2
       P    11 ± 4
       R    87 ± 1
      A     88 ± 2
      L     23 ± 3
      G     83 ± 1
      K     14 ± 2
      D     10 ± 0
      F     7 ± 3
     E      91 ± 4
     G      91 ± 3
     L      83 ± 5
     W      92 ± 1
    A       83 ± 2
    R       13 ± 4
    L       53 ± 2
    E       57 ± 2
    P       67 ± 2
  A         94 ± 2
  G         60 ± 3
  R         11 ± 4
A          80 ± 3
E          9 ± 0
K          8 ± 3
I         G 91 ± 2
      A A    91 ± 2
A      A     69 ± 5
A A         60 ± 5
A A A A       28 ± 4
  1. aPercent NS1-2A cleavage was calculated as described in the Materials and Methods; mean values of at least 3 independent experiments ± the standard error of the mean (SEM) are given