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Table 1 Effect of mutations introduced into the MVEV octapeptide and at the P1’ residue on NS1-2A cleavage

From: Proteolytic cleavage analysis at the Murray Valley encephalitis virus NS1-2A junction

P8

P7

P6

P5

P4

P3

P2

P1

P1’

% cleavagea (mean ± SEM)

L

V

K

S

R

V

Q

A

F

88 ± 1

        

Y

84 ± 0

        

V

80 ± 5

        

G

86 ± 3

       

S

 

53 ± 2

       

L

 

6 ± 1

       

F

 

7 ± 0

       

R

 

7 ± 0

       

D

 

10 ± 1

      

A

  

88 ± 2

      

Y

  

79 ± 3

      

G

  

37 ± 4

      

D

  

89 ± 4

      

L

  

89 ± 2

      

P

  

11 ± 4

      

R

  

87 ± 1

     

A

   

88 ± 2

     

L

   

23 ± 3

     

G

   

83 ± 1

     

K

   

14 ± 2

     

D

   

10 ± 0

     

F

   

7 ± 3

    

E

    

91 ± 4

    

G

    

91 ± 3

    

L

    

83 ± 5

    

W

    

92 ± 1

   

A

     

83 ± 2

   

R

     

13 ± 4

   

L

     

53 ± 2

   

E

     

57 ± 2

   

P

     

67 ± 2

 

A

       

94 ± 2

 

G

       

60 ± 3

 

R

       

11 ± 4

A

        

80 ± 3

E

        

9 ± 0

K

        

8 ± 3

I

       

G

91 ± 2

     

A

A

  

91 ± 2

A

    

A

   

69 ± 5

A

A

       

60 ± 5

A

A

A

A

     

28 ± 4

  1. aPercent NS1-2A cleavage was calculated as described in the Materials and Methods; mean values of at least 3 independent experiments ± the standard error of the mean (SEM) are given
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Virology Journal

ISSN: 1743-422X