Fig. 7

Detection of BYDV GAV in field aphids by dot-ELISA and RT-PCR. a, Detection of BYDV GAV in aphids through dot-ELISA. Row a1-7 to d1-7, and Row e 1–5 were 1–33 field aphids shown in the panel B. Row e 6 (CK-) and 7 (CK+) were from the non-viruliferous and viruliferous aphid, respectively, and used as controls. The membrane was probed with the monoclonal antibody 18A1 followed by the goat anti-mouse IgG/HRP conjugate. Blue color indicated a positive reaction. Light to dark brown color indicated a negative reaction. b, Detection of BYDV GAV in 33 field collected aphids by RT-PCR. Lane M, 1Kb DNA marker